Isolation, purification and in vitro differentiation of cytotrophoblast cells from human term placenta
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چکیده
منابع مشابه
Isolation, purification and in vitro differentiation of cytotrophoblast cells from human term placenta
BACKGROUND The syncytialization of cytotrophoblast cells to syncytiotrophoblast is central to human placental transport and hormone production. Many techniques for in vitro study of this process have been proposed and new investigators to the field may find the literature in the field daunting. Here, we present a straightforward and reliable method to establish this important model using modern...
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Differentiation of the human placental trophoblast cell involves a multistep process, with the generation of several distinct types of intermediate cytotrophoblast cells. Using a short term in vitro cell culture system and centrifugal elutriation, we studied the isolation and morphological and biochemical differentiation of these separated intermediate cell populations. Freshly isolated cell fr...
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ژورنال
عنوان ژورنال: Reproductive Biology and Endocrinology
سال: 2015
ISSN: 1477-7827
DOI: 10.1186/s12958-015-0070-8